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KMID : 0613820020120040469
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Journal of Life Science
2002 Volume.12 No. 4 p.469 ~ p.476
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Up-regulation of Bax is associated with DNA topoisomerase I inhibitor β-lapachone-induced apoptosis in human prostate carcinoma cells
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Kong kyu-Ri
Choi Byung-Tae
Choi Yung-Hyun
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Abstract
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The DNA topoismerase I inhibitor β-lapachone, the product of a tree from South America, is known to exhibit various biological properties, however the mechanisms of which are poorly understood. In the present report, we investigated the effects of β-lapachone on the growth of human prostate carcinoma DU-145 cells. Upon treatment with β-lapachone, a concentration-dependent inhibition of cell viability was observed and cells developed many of the hallmark features of apoptosis, including condensation of chromatin and DNA fragmentation. Flow cytometry analysis confirmed that β-lapachone increased populations of apoptotic-sub G1 phase. In addition, proteolytic cleavages of poly (ADP-ribose) polymerase (PARP) and β-catenin protein were observed after treatment of β-lapachone. These apoptotic effects of β-lapachone in DU-145 cells were associated with marked induction of Bax protein, however the levels of Bcl-2 expression were decreased in a dose-dependent manner
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KEYWORD
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β-lapachone, prostate carcinoma, apoptosis, Bax, Bcl-2
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